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KMID : 0379520040200030241
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한국독성학회지
2004 Volume.20 No. 3 p.241 ~ p.250
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Bisphenol A Disturbs Intracellular Calcium Homeostasis and its Relationship with Cytotoxicity
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Lee Yuk-Mo
Lee Sang-Min
Son Dong-Joo
Lee Sun-Young
Park Hye-Ji
Nam Sang-Yoon
Kim Dae-Joong
Yun Young-Won
Yoo Hwan-Soo
Oh Ki-Wan
Kim Tae-Sung
Han Soon-Young
Hong Jin-Tae
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Abstract
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We previously found that bisphenol A (BPA) caused neurotoxic behavioral alteration. Since disturbance of calcium homeostasis is an implicated contributor in the neurotoxic mechanism of environmental toxicants, we investigated whether BPA alters calcium homeostasis. Unlike other neurotoxic agents which cause increase of intracellular calcium level, BPA decreased [Ca^{2+}]_i dose-dependently in PC12 cells and cortical neuronal cells regardless of the calcium existence in buffer. BPA at greater concentrations than 100 mutextrm{M} reduced cell viability significantly in both types of cells. BPA also suppressed L-glutamate (L-type channel activator, 30 mM) and trifluoperazine (calmodulin antagonist, 30 mutextrm{M})-induced increase of [Ca^{2+}]_i. BPA further lowered caffeine (RYR activator, 100 mutextrm{M})-decreased [Ca^{2+}]_i, but did not alter dantrolene (RYR inhibitor, 100 mutextrm{M}), heparin (IP3 inhibitor, 200 units/ml) and xestospongin C (IP3 inhibitor, 5 mutextrm{M})-decreased [Ca^{2+}]_i. Cell viability was not directly related to intracellular calcium change by bisphenol A that alternation of intracellular calcium may not be a direct causal factor of BPA-induced neuronal cell death.
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KEYWORD
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Sisphenol A, Calcium homeostasis, Neurotoxic mechanism
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